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Title : Dr.
First Name : RUBY
Last Name : SHARMA
University/Institution : Albert Einstein College of Medicine
Phone # : +13478335074
Email ID : ruby.sharma@einsteinmed.org
City : Bronx
Country : United States
State : New york
Zipcode : 10461
Department : Microbiology and Immunology
Company Name :
Area of Research
Biochemistry and Structural Biology
Area of Expertise
Biochemical, BioPhysical and biological characterization of different proteins
Brief Description of Research Interest :

Microbiology: Proficient with aerobic and anaerobic techniques for screening, isolation, strain characterization and development, other related microbial assays and microscopy.

Molecular biology: Confident in various molecular biology techniques like Gene cloning, primer designing, polymerase chain reaction, isolation of genomic DNA, plasmid DNA, RNA, Restriction-digestion, transformation, competent cells preparation, analysis of DNA, RNA and protein, Co-Immuno Precipitation.

Protein Science: Specialized training in Recombinant protein expression and purification using Chromatographic means like Affinity(Ni -NTA, MBP and GST), Size exclusion, Hydrophobic and Ion-exchange Chromatography, salting out, Refolding of proteins.

Macromolecular Crystallography: Enthusiastic Protein crystallographer acquainted with crystallization using hanging drop and sitting drop methods manually as well as using robotics (mosquito from TTP Labtech). Protein-Ligand complex crystallization, Crystal handling, mounting, Crystal soaking, Data collection at home source on BRUKER Microstar and Rigaku Micromax (CuK-α) and also on Synchrotron Radiation source (ESRF-BM14), Grenoble, France. 

Other Biochemical and Biophysical techniques: Managed Projects including Enzymatic assays using spectrophotometer, ELISA reader and kinetic studies of enzymes.

Went the extra mile by performing biophysical techniques like Estimation of various bio-molecules(Protein, DNA, lipid, Carbohydrate etc), Fluorescence spectroscopy, DLS, CD,ITC, SPR, XRD, SAXS, MALS and GC-MS.

Representative Publications :

1. Bhumika Kumar, Sonia Verma, Mohammad Kashif, Ruby Sharma, Atul, Rajnikant Dixit, Agam P. Singh, Ajay K. Saxena, Mohammad Abid, Kailash C.Pandey*. Metacaspase-3 of Plasmodiumfalciparum: An atypical trypsin-like serine protease. (Oct, 2019) International Journal of Biological Macromolecules Vol 138, 309-320.

2. Rahul Pasupureddy, Sonia Verma, Akansha Pant, Ruby Sharma, Sriram Seshadri, Veena Pandey, Ajay Kumar Saxena, Rajnikant Dixit and Kailash C Pandey. Crucial residues in falcipains that mediate hemoglobin hydrolysis. (Feb, 2019) Experimental Parasitology.Vol197, 43-50.

3.Ruby Sharma, Shanti P. Gangwar, AjayK. Saxena. Comparative structure analysis of ETSi domain of ERG3 and itscomplex with E74 promoter DNA sequence (2018) Acta Crystallographica Section F. Vol. F74,656-663.

4.Akansha Pant, Rajendra Kumar, Naiem Ahmed Wani, Sonia Verma, Ruby Sharma,Supriya Sharma, Veena Pande, Ajay Kumar Saxena, Rajnikant Dixit, Raj KishoreRai and Kailash C. Pandey (2018). Allosteric Site Inhibitor DisruptingAuto-processing of Malarial Cysteine Proteases Nature Scientific Reports; Vol. 8: 16193.

5. Vandana, Agam Prasad Singh, Jitendra Singh, Ruby Sharma, Mymoona Akhter, Ajay Saxena, Anju Katyal, Rajnikant Dixitand Kailash C Pandey. (2018) Biochemical Characterization of Unusual Cysteine Protease of P. falciparum,Metacaspase-2 (MCA-2). Molecular & Biochemical Parasitology. Vol.220, 28-41.

6. Kavita Pahuja, Adil Beg, Ruby Sharma, Ajay Kumar Saxena, NitiPuri and Angamuthu Selvapandiyan (2018) Importance of a novel trypanosome specific glycosomal protein for the cell cycle of Leishmaniadonovani parasites  BBA-Molecular Cell Research. 1865:Vol. 8,  1148-1159.

7. Srinivasan Sundararaj, Ajay K. Saxena, Ruby Sharma, Kapil Vashisht, Supriya Sharma, Anup Anvikar, Rajnikant Dixit, Philip J. Rosenthal, Kailash C. Pandey. (2014) Cross-Talk between Malarial Cysteine Proteases and Falstatin: The BC Loop as a Hot-Spot Target.  Plos One. Vol 9,  Issue 4 , e93008.

8.Manoj Kumar, Ruby Sharma, Meenakshi Dua, Narendra Tuteja and Atul KumarJohri (2013) .Development of Transformation System for RootEndophytic Fungus Piriformospora indica  Chapter published in Wiley-Blackwell.

9. Manoj Kumar, RubySharma, Abhimanyu Jogawat, Pratap Singh, Meenakshi Dua, Sarvajeet SinghGill, Dipesh Kumar Trivedi, Narendra Tuteja, Ajit Kumar Verma, Ralf Oelmullerand Atul Kumar Johri. (2012) Piriformospora indica, A Root Endophytic Fungus, Enhances AbioticStress Tolerance of The Host Plant published in Wiley-Blackwell, Improving Crop Resistance to Abiotic Stress, (Chapter )Vol. 1, 543-558.

10. Manoj Kumar, Vikas Yadav, Hemant Kumar, Ruby Sharma, Archana Singh, Narendra Tuteja and Atul Kumar Johri (2011). Piriformosporaindica enhances plant growth by transferring phosphate. Plant Signalling and Behaviour (PSB). Vol. 6: 723-725.

11.Dinesh Kumar, Rachna Verma, Priyanka Sharma, Ankush Rana, Ruby Sharma,Chandan Prakash and Tek Chand Bhalla (2010). Production and partialpurification of xylanase from a new thermophilic isolate. Biological Forum —An International Journal, Vol. 2(2): 83-87.

12. Vikas Yadav, Manoj Kumar, Deepak Kumar Deep, Hemant Kumar, RubySharma, Takshashila Tripathi, Narendra Tuteja, Ajay Kumar Saxena, and Atul Kumar Johri (2010). APhosphate Transporter from the root endophytic fungus Piriformospora indicaplays a role in phosphate transport to the host plant. The Journal of Biological Chemistry, (JBC). Vol. 285: 26532-26544.

(In Preparation)

13. Ruby Sharma, Chacko Jobichen, Kunchithapadam, Swaminathan and Ajay K. Saxena. Crystal structure of humanERG3-cfosDNA complex (In Preparation).

14. Vipin K. Kashyap1*, Ruby Sharma1*, Manoj Kumar2, Abhisheka Bansal2 and Ajay K. Saxena1#(Equal contribution). Dissection of function and recognition mechanism of M. tuberculosis ESX-1secreted virulence factor EspC (In Preparation).

15. Yeshveer Singh, Ruby Sharma, Praveen Kumar Verma,Ajay Kumar Saxena. Structural and Functional Analysis of the ArOYE6 enzyme from necrotrophic fungus Aschochyta rabeiei (In Preparation).

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