MEMBER PROFILE
| First Name | Nadine |
| Last Name | Koertel |
| University/Institution | University of Colorado, Anschutz Medical Campus |
| Email ID | [email protected] |
| City | Aurora |
| Country | United States |
| State | Colorado |
| Zip code | 80045 |
| Department | Biochemistry and Molecular Genetics |
| Area of Research | RNA Biology, Epitranscriptomics, Cancer Biology |
| Area of Expertise | RNA Biology, Epitranscriptomics, Cancer Biology, NGS, Illumina sequencing, Nanopore sequencing |
Brief Description of Research Interest :
Since the early beginnings of my academic career, my research has established my long-standing interest in the epitranscriptome with its biological consequences. This provided an excellent background in different disciplines such as molecular biology, RNA biology, high-throughput sequencing methods, as well as behavioral and molecular genetics techniques.
During my Masters, I uncovered the potential evolutionary conserved function of the pseudouridine synthetase pus7 in cognition and neuronal development in Drosophila melanogaster, which was featured in a shared first author publication. During my PhD, I was part of the International PhD Program at the Institute of Molecular Biology in Mainz, Germany in the lab of Dr. Julian König. My work focused on the development of high-throughput sequencing methods to globally map the N6- methyladenosine RNA modification, and to unravel novel functions. We combined our recently improved iCLIP2 protocol with the miCLIP procedure to allow efficient mapping of m6A modifications. We further uncovered a novel function of m6A in X-to-autosome dosage compensation in mammals. Combined, this work led to three publications, including two first author publications. I gained an excellent expertise with deep knowledge in various high-throughput sequencing methods and RNA biology. During my postdoctoral work, I'm building on my expertise while transferring my research to a translational research context in cancer.
Representative Publications:
https://scholar.google.com/citations?user=BtPq2DMAAAAJ&hl=en
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Körtel N, Rücklé C, Zhou Y, Busch A, Hoch-Kraft P, Sutandy FXR, Haase J, Pradhan M, Musheev M, Ostareck D, Ostareck-Lederer A, Dieterich C, Hüttelmaier S, Niehrs C, Rausch O, Dominissini D, König J, Zarnack K. Deep and accurate detection of m6A RNA modifications using miCLIP2 and m6Aboost machine learning. Nucleic Acids Res. 2021 Sep 20;49(16):e92. doi: 10.1093/nar/gkab485. PMID: 34157120; PMCID: PMC8450095.
Rücklé, C., Körtel, N., Basilicata, M.F. et al. RNA stability controlled by m6A methylation contributes to X-to-autosome dosage compensation in mammals. Nat Struct Mol Biol 30, 1207–1215 (2023). https://doi.org/10.1038/s41594-023-00997-
de Brouwer APM, Abou Jamra R, Körtel N, Soyris C, Polla DL, Safra M, Zisso A, Powell CA, Rebelo-Guiomar P, Dinges N, Morin V, Stock M, Hussain M, Shahzad M, Riazuddin S, Ahmed ZM, Pfundt R, Schwarz F, de Boer L, Reis A, Grozeva D, Raymond FL, Riazuddin S, Koolen DA, Minczuk M, Roignant JY, van Bokhoven H, Schwartz S. Variants in PUS7 Cause Intellectual Disability with Speech Delay, Microcephaly, Short Stature, and Aggressive Behavior. Am J Hum Genet. 2018 Dec 6;103(6):1045-1052. doi: 10.1016/j.ajhg.2018.10.026. PMID: 30526862; PMCID: PMC6288278.
Zhou Y, Ćorović M, Hoch-Kraft P, Meiser N, Mesitov M, Körtel N, Back H, Naarmann-de Vries IS, Katti K, Obrdlík A, Busch A, Dieterich C, Vaňáčová Š, Hengesbach M, Zarnack K, König J. m6A sites in the coding region trigger translation-dependent mRNA decay. Mol Cell. 2024 Dec 5;84(23):4576-4593.e12. doi: 10.1016/j.molcel.2024.10.033. Epub 2024 Nov 21. PMID: 39577428.